In the fall I give a lecture to incoming neuroscience graduate students on how to perform light microscopy in a rigorous and reproducible manner. I wanted to share that presentation here for accessibility.
The introduction of conditional genetics represents a huge boon in the health sciences. It used to be that the only way to study the effect a gene had on the biology of a system was to “knock it out” - engineer a model (usually mouse) where the gene, or part of the gene, is missing entirely from the mouse’s genome.
Have you ever wondered how we make these bright and beautiful images of proteins in the brain? It’s done using a technique called immunohistochemistry, which we also call IHC or immuno.
Preprint featuring one of the projects I worked on during my time as a research assistant! Here we start to put together the molecular signaling pathway downstream of non-ionotropic LTD and spine shrinkage.